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. 2014 May 2;289(24):16924–16935. doi: 10.1074/jbc.M113.542795

FIGURE 7.

FIGURE 7.

TAT-p27 reduced apoptosis post-MI by promoting autophagy. A and B, homogenates from mice undergoing sham and LAD ligation surgery, with the latter receiving treatments with TAT-p27 or TAT-β-Gal alone or in combination with the autophagy inhibitor CQ, were probed for LC3-II, p62, and cleaved caspase 3. β-Tubulin served as loading control. Compared with sham-operated controls, post-MI groups revealed higher levels of LC3-II and cleaved caspase 3 levels (*, p < 0.05 versus sham). Levels of p62 were more varied. Compared with TAT-β-Gal-treated controls, TAT-p27-treated hearts revealed higher levels of LC3-II and lower levels of p62, consistent with increased autophagy. TAT-p27-treated hearts also revealed lower levels of cleaved caspase 3 than TAT-β-Gal-treated controls, consistent with reduced apoptosis (#, p < 0.05 versus TAT-β-Gal). In the presence of CQ, treatment with TAT-p27 failed to reduce expression of the apoptosis marker cleaved caspase 3 versus TAT-β-Gal-treated controls (p = not significant).