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Porcn can transfer a fatty acid to either Ser or Thr at position 209 in Wnt3a. A, COS-1 cells expressing WT Porcn and the indicated Wnt3a constructs were labeled and analyzed as in Fig. 1b. The experiment was repeated two times in duplicate; a representative image is shown. B, relative luciferase activity for the Wnt3a constructs in A was determined as in Fig. 4C. IP, immunoprecipitation; WB, Western blot.
