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. 2014 May 2;289(24):17174–17183. doi: 10.1074/jbc.M113.546135

FIGURE 1.

FIGURE 1.

α1-AR signaling up-regulated the transcriptional factor Nfil3 in MC3T3-E1 cells. A, Nfil3 mRNA expression in MC3T3-E1 cells. Cells were treated with 1 μm PHE for 1, 2, and 4 h, harvested, and then processed for real-time qRT-PCR. Each value represents the mean ± S.E. of six separate experiments. *, p < 0.05, significantly different from each control value obtained in MC3T3-E1 cells cultured in the absence of PHE. B, Nfil3 mRNA was up-regulated by PHE in a concentration-dependent manner in MC3T3-E1 cells. Cells were treated with PHE at 0.1–10 μm for 2 h, harvested, and processed for real-time qRT-PCR. Each value represents the mean ± S.E. of six separate experiments. *, p < 0.05, significantly different from each control value obtained in MC3T3-E1 cells cultured in the absence of PHE. C, the transcriptional factor Nfil3 was increased by PHE in a dose-dependent manner in MC3T3-E1 cells. Cells were treated with PHE at 0.1–10 μm for 4 h, harvested, and then processed for Western blotting. A representative result of three individual experiments is shown.