FIGURE 2.

α1-AR signaling mediated Nfil3 mRNA expression after the PHE stimulation in MC3T3-E1 cells. Cells were incubated for 2 h in the presence of PHE with prazosin at a concentration of 10 μm, followed by the determination of Nfil3 levels by real-time qRT-PCR. Each value represents the mean ± S.E. of three separate experiments. *, p < 0.05, significantly different from each control value. NS, not significant.