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Nfil3 and Bmp4 in bone. A representation of the expression of Nfil3 and Bmp4 in bone from C57BL/6J mice under light/dark cycle conditions. Bone was obtained from C57BL/6J mice every 4 h. Total RNA was isolated, and the level of mRNA was determined by real-time qRT-PCR using specific primers for Nfil3 and Bmp4. Relative mRNA levels were normalized to the Gapdh level. Data represent the mean ± S.E., n = 6–8 animals. White boxes, light period; black boxes, dark period.
