FIGURE 5.

Nfil3 negatively regulated Per2 and Bmp4 in MC3T3-E1 cells. Effects of the overexpression of Nfil3 in MC3T3-E1 cells. MC3T3-E1 cells were stably transfected with expression vectors for Nfil3 (pcDNA-Nfil3) or control (pcDNA-Negative), followed by further cultivation for 48 h and the subsequent determination of Per2, Bmal1, and Bmp4 levels by real-time qRT-PCR. Each value represents the mean ± S.E. of four separate experiments. *, p < 0.05, significantly different from the value obtained in cells transfected with the control vector.