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. 2014 May 2;289(24):17174–17183. doi: 10.1074/jbc.M113.546135

FIGURE 7.

FIGURE 7.

Deletion of putative D-box elements abolished Nfil3-mediated suppression of Bmp4 luciferase activity. A, MC3T3-E1 cells were transiently transfected with Bmp4-Luc in either the presence or absence of the Nfil3 expression plasmid. Luciferase activity was determined 48 h after transfection. The total amount of DNA transfected was standardized with an empty vector. Firefly luciferase activities were normalized by Renilla luciferase activities. Each value is the mean ± S.E. (n = 5–6 independent experiments). *, p < 0.05, significantly different from each control value. B, MC3T3-E1 cells were transfected with ΔDboxBmp4-Luc in either the presence or absence of the Nfil3 expression plasmid. The luciferase activity was determined 48 h after transfection. The total amount of DNA transfected was standardized with an empty vector. Firefly luciferase activities were normalized by Renilla luciferase activities. Each value is the mean ± S.E. (n = 5–6 independent experiments).