FIGURE 2.

Tyr⁵¹⁰ is an important site of phosphorylation in Dbl. A, domain structure of the Dbl protein. Of the 29 tyrosines of Dbl, the top-scoring putative phosphorylation sites are indicated, as well as the immediate sequence surrounding Tyr⁵¹⁰. B, tyrosine phosphorylation of Dbl(Y510F) was examined as in Fig. 1A. Data are representative of three independent experiments. PY, phosphotyrosine; IB, immunoblot; DH, Dbl homology domain; PH, pleckstrin homology domain; SPEC, spectrin.