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. 2014 Apr 24;289(24):17215–17227. doi: 10.1074/jbc.M113.536664

FIGURE 2.

FIGURE 2.

AS101 injection reduces clinical symptoms of DSS-induced murine colitis. Colitis was induced by addition of 3.5% DSS to the drinking water of C57Bl/6 mice. AS101 (10 μg/mouse) was injected daily intraperitoneally from day 0 (DSS+AS(d0)) or from 2 days after DSS administration (DSS+AS(d2)). A, AS101 restores decreased body weight induced by colitis. Total average body weight of each group was monitored. *, p < 0.01 decrease versus other groups (analyzed on days 6–11 by ANOVA repeated measures). B, AS101 reduces intestinal bleeding induced by colitis. Intestinal bleeding was scored by Hemoccult test for the presence of occult blood and by visual observation of rectal bleeding. *, p < 0.001 increase versus other groups (analyzed by ANOVA repeated measures). C, AS101 reverses colitis-induced elevation of stool morphology score. *, p < 0.001 increase versus other groups (analyzed by ANOVA repeated measures). D and E, AS101 restores colon length shortened by colitis. Colons were removed and measured at necropsy (day 12). *, p < 0.001 increase versus DSS+PBS group (analyzed by one-way ANOVA test). The results shown are means ± S.E. (n = 4 for PBS; n = 8 for DSS+PBS; n = 6 for DSS+AS(d0); n = 10 for DSS+AS(d2)). For colon length, n = 7 for PBS; n = 18 for DSS+PBS; n = 17 for DSS+AS(d0); n = 10 for DSS+AS(d2).