FIGURE 6.

AS101 prevents neutrophil migration into the colon. Colitis was induced by addition of 3.5% DSS to the drinking water of C57Bl/6 mice. AS101 (10 μg/mouse per injection) was injected from day 0 or starting 2 days after DSS administration. At necropsy (day 7), 2 cm of the distal colon was removed and cut longitudinally. One section was fixed in 4% buffered formaldehyde and stained with hematoxylin and eosin. The other section was kept at −80 °C until assay for MPO activity, as described under “Experimental Procedures.” A, histopathological examination of the distal colon. The pictures shown are representative of three mice per group. Scale bars indicate 160 μm. B, MPO activity in the distal colon. *, p < 0.05 decrease versus DSS+PBS group (analyzed by Students t test). The results shown are means ± S.E. (n = 8 for PBS; n = 7 for DSS+PBS; n = 8 for DSS+AS(d0); n = 9 for DSS+AS(d2)).