FIGURE 9.

AS101 prevents the migration of CD4⁺ cells into the colon and increases colonic CD4⁺CD25⁺Foxp3⁺ T regulatory cells. A, colitis was induced by addition of 3.5% DSS to the drinking water of C57Bl/6 mice. AS101 (10 μg/mouse per injection) was injected daily intraperitoneally from day 0 (DSS+AS(d0)) or from 2 days after DSS administration (DSS+AS(d2)). At necropsy (day 7), 1–2 cm of the distal colon was removed and fixed in 4% buffered formaldehyde. Immunohistochemical analysis was performed in paraffin-embedded slides. The pictures shown are representative of three mice per group. Scale bars indicate 160 μm. B, lamina propia lymphocytes cells were isolated from the colons of treated groups and stained for CD4⁺CD25⁺Foxp3⁺ T regulatory cells as described under “Experimental Procedures.” Dot plots of each group showing CD25⁺FOXP3⁺ LPL cells. (Plots are gated on CD3⁺CD4⁺ LPL cells.) Each plot is representative of three experiments.