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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Nat Commun. 2013;4:1515. doi: 10.1038/ncomms2526

Figure 4. Radiation-induced loss of salivary fluid secretion is absent in TRPM2−/− mice.

Figure 4

(a) H2O2-induced increases in [Ca2+]i in SMG acini from TRPM2+/+ and TRPM2−/− mice. (b) IR-induced increase in constitutive Ca2+ influx in TRPM2+/+ and TRPM2−/− mice. (c) Average data from the experiments shown in a and b. **Indicates values with statistically significant difference compared with the unmarked values in each experiment (number of cells tested from 175–205 per group). Mice were treated with the muscarinic agonist, pilocarpine (0.5mg kg−1, subcutaneous injection) and saliva flow was collected (details given in the Methods section) in TRPM2+/+ and TRPM2−/− mice before IR (d shows saliva secretion over a 20-min collection period), on different days after 15 Gy IR (e up to 120 days); and over a 20-min time point 30 days after IR (f). **Indicates significant difference, P < 0.01, between the corresponding values in TRPM2+/+ mice (10–11 animals per group). All values in (c–f) are defined as mean ± s.e.