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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Clin Cancer Res. 2014 Mar 21;20(10):2684–2694. doi: 10.1158/1078-0432.CCR-13-3268

Figure 2. CMV pp65 RNA-pulsed mature dendritic cells induce robust activation of CMV pp65-specific CD8+ T cells.

Figure 2

T cells were stimulated ex vivo with autologous CMV pp65 RNA-pulsed DCs using PBMCs from 1 normal donor and 2 GBM patients. T cells were analyzed pre- and post-stimulation for the presence of CD8+ and CMV pp65 tetramer+ cells using flow cytometry as described in Methods. CMV pp65 HLA-A*0201 (normal and Patient 1) and HLA-B*3501 (Patient 2) PE-labeled tetramers were used.