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. 2014 May 19;30(22):6629–6635. doi: 10.1021/la500822w

Figure 5.

Figure 5

Comparison of epoxy-modified (blue) and an azido-modified (red) slides. Part a: slides were treated with 0.1–100 μM proL-CVIApf at rt for 2 h and incubated with mouse anti-goat IgG (55 μL, 1 mg/mL) and Alexa 680-labeled goat anti-rabbit IgG (1.0 μg/mL) at rt for 1 h. The slides were visualized and treated with Alexa 680-labeled goat anti-rabbit IgG (excitation/emission at 633/670 nm). The slides were treated with 0.1 M glycine at pH 2.6 for 40 min, and the treatment/visualization steps were repeated. Part b: plot of relative fluorescence intensity versus [proL-CVIApf].