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Multiple reuse of proL-CVIApf in sandwich-type antibody array. The slides were immobilized with 100 μM proL-CVIApf at rt for 2 h and subsequently incubated with mouse anti-GFP IgG (55 μL, 1 mg/mL), GFP (0.01–10 μM), and DyLight 680-labeled mouse anti-GFP IgG (1.0 μg/mL) at rt for 1 h. Fluorescence intensities were measured by each excitation/detection at 532/526 nm (GFP) and 633/670 nm (DyLight 680-labeled goat anti-GFP IgG).
