Figure 5.

The cen1::2Bi embedded reporter genes are unstable. (A) Frequency of ura4⁺ arg3⁺ auxotrophy in indicated strains. The dotted line for each strain represents the mean and the error bars indicate the standard deviation. Data points are independent biological replicates. (B) ChIP-qPCR average relative enrichment of CENP-A^Cnp1 in wild type (WT), cen1::2Bi, and cen1::2Bi revertants at locus 3, illustrated in Figure 4A. Wild-type and cen1::2Bi data are identical to data in Figure 4C. Each light blue bar represents an independently isolated revertant strain, for which a single ChIP-quantitative polymerase chain reaction experiment was performed.