Figure 2.

11R2 is an allele of nudE: overview of the single-nucleotide polymorphism (SNP) mapping. (A and F) grk*mCherry in 11R2 (A) and nudE^39A (F) stage 3−6 egg chambers. Lack of localized grk*mCherry indicates the absence of oocyte. (B) Lack of Orb staining in a 11R2 germline clone (*, marked by the absence of LacZ expression; B′) confirms the failure to form an oocyte. Shown is a projection of a series of z-stack confocal sections that cover the whole egg chamber. (C) Crossing scheme for the SNP mapping. Individual F2 females are mated to the tester males (as in the primary screen), and F3 progeny are used for phenotyping (in germline clones) and genotyping. (D, E) summary of SNP genotyping. (D) 48 putative recombinants were genotyped for the SNPs at 61A and at 75F. The ratio of mutant and wild-type recombinants in each group yields an approximate map position. Nonrecombinant lines (group 3) are excluded from the subsequent assays. (E) Genotyping by exclusion shows that the causative lesion lies between 66F and 69C. If necessary, further mapping could have been performed using the four remaining recombinants. Scale bars = 20 μm.