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. 2014 Feb 14;4(4):749–760. doi: 10.1534/g3.114.010462

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Copyright © 2014 Hayashi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phenotypes of other mutants identified in the screen. (A) grk*mCherry localizes to the center of the qm^11S5 egg-chamber (arrow). (B−B′′) Clustering of ring canals in center of qm^5R4 oocytes as revealed by coincident central staining of mutant oocyte for F-actin (phalloidin, B), Dynein heavy chain (Dhc, B′), and grk*mCherry (grk, B′′). The lack of F-actin staining between nurse cell nuclei suggests that the cell membranes have collapsed. (C) Staining of F-actin (phalloidin) in qm^L14.4 germline clone. (D) Laterally mispositioned oocyte (Oo) in a Tsc2^2R5 mutant stage 7−9 egg-chamber. (E, F) Phalloidin staining of wild-type (E) and Chd5^13P2 (F) stage 10B egg-chambers showing discontinuous actin filaments (arrows in F′) and a nurse cell nucleus (asterisk) going beyond the border of nurse cells and the oocyte. A different z-section of the same egg-chamber shows a collapsed ring canal (arrow in F′′). (G, H) Grk protein is more diffuse in the cytoplasm of rasp^13P10 (H) than wild-type (G) stage 8−9 egg-chambers. scale bars = 20 μm.