Figure 7.

Disrupted centrosome clustering in klc and piRNA pathway mutant oocytes reveals two modes of microtubule organizations involved in grk mRNA localization. Costaining of grk*mCherry (grk) and centrosome components Asterless (Asl, A, B, D-H) or γ-Tubulin (γ-Tub, C, I) in wild-type (A, D, I), and klc^sat (B, C, E), mael^sat (F), saturn (G), and armi^3R13 (H) germline clones. D′-H′ are the enlarged images of selected square in D−H, respectively. Centrosome components are dispersed both in klc (B, C, and E) and piRNA pathway mutants (mael in F′ and armi in H′) and are internally misplaced in klc mutant, like grk mRNA (E). Arrows indicate faint centrosomes deriving from follicle cells (E). Centrosome components are more dispersed in saturn (G′) oocyte than in klc (E’) and mael (F′) mutant oocytes alone. (I) Snapshots of stage 7 wild-type egg-chambers, showing that dorsal-anterior localization of grk mRNA precedes the centrosome migration. Scale bars = 10 μm.