Table 1.
Mutants identified from the screen
| gene | alleles | grk mRNA localization in stage 6 | grk mRNA localization in stage 9 | other phenotypes | |
|---|---|---|---|---|---|
| mislocalised grk mRNA in late stages | armitage (armi) | 3R13, 9R1, 10M12, 11R3, 11S9, 13P2, 13P5, 14P4 | diffuses internally | weakly accumulates around the nucleus, spreads along anterior periphery | defective follicle cell development |
| saturn*: kinesin light chain (klc) & maelstrom (mael) | 5R12 | diffuses internally | no longer localizes to the anterior periphery nor to the nucleus | mispositioned nucleus | |
| scaf6 + unmapped mutation | 12M9 | diffuses internally | weakly accumulates around the nucleus, spreads along anterior periphery | defective reproduction of germline stem cells | |
| elg1 | 3R7 | localizes posteriorly as in wild type | spreads along anterior periphery | defective nurse cell DNA endoreplication |
| Phenotypes | |||||
| other mutants | nudE | 11R2 | Oocyte is misspecified. Most egg chambers contain 16 nurse cells. | ||
| quemao (qm) | 5R4, 11S5 | Plasma membranes of nurse cells and the oocyte collapse. Released ring canals cluster. grk mRNA colocalizes to the ring canal cluster. | |||
| Tsc2/gigas | 1R8, 2R5, 11R10 | Oocyte is frequently mispositioned. | |||
| rasp | 11R5, 13P2, 13P10, 13P13 | Mature eggs have fused appendages. Gurken proteins diffuse in the cytoplasm. | |||
| Chd5/Wrb | 9R9, 13P2 | Disorganized nurse cells in late stage 10. | |||
*
saturn (5R12) is a synthetic phenotype caused by mutations in klc and mael (see Results).