Table 3. Molecular lesions of armitage alleles.
Allele Name | Amino Acid Change | Domain | grk Mislocalization | Eggs Laid by Hemizygous Females |
---|---|---|---|---|
3R13, 9R1 | Q315 > stop | C-terminal truncation | + | – |
11R3 | G583 > D | Unknown domain | + | – |
14P4 | Q674 > stop | C-terminal truncation | + | – |
13P2 | G728 > E | Helicase domain | + | – |
10M12 | Splicing donor of intron 7 (GT to AT) | Exon 8 skipped, which truncates after aa 1082 | + | – |
11S9 | E1082 > K | Helicase domain | + | + |
13P5 | R1121 > stop | C-terminus of helicase domain truncated | + | – |
The helicase domain lies between amino acids 698−1132 of the 1188 amino acids Genbank accession no ABX00729.1. Df(3L)E1 was used to make flies hemizygous for each allele.