Figure 6.
Effect of 1 on PfAtg8 protein levels. (A) Dose-dependent immunoblot analysis of P. falciparum treated with DMSO or 3.375, 6.75, 12.5, or 25 μM 1 for 6 h. Chloroquine (CQ) at 50 nM was used as a positive control of autophagy inhibition. Atg8-PE has a faster migration than unlipidated Atg8 with SDS-PAGE. Arrows indicate the migration of lipidated and unlipidated PfAtg8. The blot was probed with antibody against TgAtg8, demonstrated to be cross reactive against PfAtg8.40 (B) Blood smears of P. falciparum after treatment with DMSO or 50 μM 1 for 5 h, observed at 100× magnification. Representative images for different stages are shown, progressing from ring stage on the left to late schizont on the right.