Figure 5.

Time for 50% release, t₅₀, calculated for microspheres supplemented with 9.9 ng mL^–1 VEGF, 0.1 ng mL^–1 [¹²⁵I]VEGF in 25 vol % serum with or without protease inhibitor (PI). Release of bound VEGF was measured in 25 vol % serum with or without PI. For each sample, logarithmic regression analysis was calculated as described in Materials and Methods, and t₅₀ values were calculated for 0.4% and 1.6% VBP and VBP_WT microspheres. Scramble and Blank microspheres were omitted from this analysis because the data could not adequately be modeled using logarithmic regression analysis. No significant differences were observed between conditions (α = 0.05).