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. 2014 Jun 16;5:258. doi: 10.3389/fpls.2014.00258

Figure 2.

Figure 2

RNP29 import is Toc 159 dependent. (A) Western blot analysis of wild type (WT) and ppi1 protoplasts transiently expressing eGFP or the indicated eGFP fusion protein containing at least the transit peptide of plastid localized FNR, RNP29, or Lhcb4. Detection was achieved by an anti-GFP antibody. The amido black stained membrane is shown as loading control. (B) Abundance of cpRNPs 29A (At3g53460, upper band) and 29B (At2g37220, lower double band) and Lhcb4.1 (At5g01530) in total protein extracts from wild type (WT) and ppi2 seedlings. In total 150 μg protein was loaded and actin was used as loading control. (C) Western blot analysis of reconstituted import of eGFP fusion proteins Fd21-100:eGFP and RNP291-100:eGFP in ppi2 protoplasts cotransformed with full length Toc159. The reporter protein was detected with an anti-GFP antibody; arrows indicate the positions of preproteins (light gray) and mature proteins (dark gray). The amido black stained membrane is shown as loading control.