Figure 3.

Import efficiency of different RNP29 constructs. The second amino acid of RNP29_1-100:eGFP (RNP29) Ala was replaced by Glu in A2E or Asn in A2N. Import efficiency of the substrates was analyzed in transiently transformed wild type (WT) and ppi2 protoplasts with different methods, showing the import efficiency of the A2E and the A2N construct. (A) CLSM of transiently transformed protoplasts, scale bars = 10 μm. (B) Western blot analysis using an anti-GFP antibody. The amido black stained membrane is shown as loading control. (C) and (D) Quantification of preprotein and mature protein using MS^E. Diagrams showing the absolute quantification with standard deviation (C) and the relation between preprotein and mature protein (D) for each construct in wild type (WT) and ppi2.