Table 4.
Antibiosis activities of the extracts and fractions from the fermentation broth of the fungus Nectria haematococca cultivated in preparative scale.
| Tested strains | Extracts | Fractions | Control | FA1 | FA2 | |||||||
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| ME | FBE | FA1 | FA2 | FA3–6 | CON | AMO | KET | MIC (μg.mL−1) | MBC (μg.mL−1) | MIC (μg.mL−1) | MBC (μg.mL−1) | |
| S. aureus | – | 23.3 ± 1.1 | 12.3 ± 0.5 | 12.3 ± 0.5 | – | – | 28.1 ± 0.28 | NT | ≥ 25 | ≥ 50 | ≥ 25 | ≥ 50 |
| E. faecalis | – | 24.3 ± 2.0 | 11.3 ± 0.5 | 11.6 ± 0.5 | – | – | 46.0 ± 1.00 | NT | ≥ 50 | – | ≥ 50 | – |
| P. avellaneum | – | 22.3 ± 2.0 | 23.3 ± 1.1 | 21.3 ± 0.5 | – | – | NT | 43.3 ± 0.6 | ≥ 12.5 | ≥ 100 | ≥ 12.5 | ≥ 100 |
ME – Ethanolic extract from the mycelium; FBE – ethyl acetate extract from the fermentation broth; FA1 – fraction from FBE obtained in dichloromethane:ethyl acetate (1:1); FA2 – fraction from FBE obtained in ethyl acetate 100%; FA3 – fraction from FBE obtained in ethyl acetate: acetone (1:1); FA4 – fraction from FBE obtained in acetone 100%; FA5 – fraction from FBE obtained in methanol 100%; FA6 – fraction from FBE obtained in methanol:water (8:2); AMO – Amoxicilin 2.0 mg.mL−1; KET – ketoconazole 2.0 mg.mL−1; CON – negative control DMSO: water (1:9); MIC – minimum inhibitory concentration; MBC – minimum bactericidal concentration; NT – not tested.