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. 2014 May 19;45(1):343–349. doi: 10.1590/s1517-83822014000100049

Table 1.

Viruses analyzed, PCRs assays, primers’ sequences, genome region and references.

Viruses and qPCR assay Primers and probe Sequences 5′ to 3′ Genome region References
RV-A RT-qPCR NSP3 F ACCATCTWCACRTRACCCTCTATGAG a NSP3 Zeng et al., 2008
NSP3 R GGTCACATAACGCCCCTATAGC
NSP3 probe VIC-AGTTAAAAGCTAACACTGTCAAA
HAdV qPCR AQ1 F GCCACGGTGGGGTTTCTAAACTT Hexon Heim et al., 2003
AQ2 R GCCCCAGTGGTCTTACATGCACATC
Probe FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA-TAMRA
NoV RT-qPCR COG2F CARGARBCNATGTTYAGRTGGATGAGa ORF1-ORF2 junction region Kageyama et al., 2003
COG2R TCGACGCCATCTTCATTCACA
RING2-probe FAM-TGGGAGGGCGATCGCAATCT-TAMRA
HAV RT-qPCR Forward primer CTGCAGGTTCAGGGTTCTTAAATC 5′ non-coding region (NC) Villar et al., 2006
Reverse primer GAGAGCCCTGGAAGAAAGAAGA
Probe FAM-ACTCATTTTTCACGCTTTCTG
a

IUB code: W = A/T, R = A/G, B = C/G/T, Y = C/T, N = A/C/G/T.