Table 1.
Viruses and qPCR assay | Primers and probe | Sequences 5′ to 3′ | Genome region | References |
---|---|---|---|---|
RV-A RT-qPCR | NSP3 F | ACCATCTWCACRTRACCCTCTATGAG a | NSP3 | Zeng et al., 2008 |
NSP3 R | GGTCACATAACGCCCCTATAGC | |||
NSP3 probe | VIC-AGTTAAAAGCTAACACTGTCAAA | |||
HAdV qPCR | AQ1 F | GCCACGGTGGGGTTTCTAAACTT | Hexon | Heim et al., 2003 |
AQ2 R | GCCCCAGTGGTCTTACATGCACATC | |||
Probe | FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA-TAMRA | |||
NoV RT-qPCR | COG2F | CARGARBCNATGTTYAGRTGGATGAGa | ORF1-ORF2 junction region | Kageyama et al., 2003 |
COG2R | TCGACGCCATCTTCATTCACA | |||
RING2-probe | FAM-TGGGAGGGCGATCGCAATCT-TAMRA | |||
HAV RT-qPCR | Forward primer | CTGCAGGTTCAGGGTTCTTAAATC | 5′ non-coding region (NC) | Villar et al., 2006 |
Reverse primer | GAGAGCCCTGGAAGAAAGAAGA | |||
Probe | FAM-ACTCATTTTTCACGCTTTCTG |
IUB code: W = A/T, R = A/G, B = C/G/T, Y = C/T, N = A/C/G/T.