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. 2014 May 19;45(1):81–88. doi: 10.1590/s1517-83822014000100012

Table 3.

Central composite experimental design using 4 variables obtained from Plackett-Burman and OVAT.

Run A: Tryptone (g.L−1) B: Soy (g.L−1) peptone C: Glucose (g.L−1) D: Citrate (g.L−1) Activity (AU.mL−1)
Units
1 20 15 20 2 3200
2 20 20 15 2 800
3 15 20 20 5 1600
4 15 15 20 2 3200
5 20 15 20 5 6400
6 20 20 20 5 3200
7 15 20 20 2 1600
8 17.5 17.5 17.5 3.5 6400
9 15 15 15 2 100
10 15 15 20 5 1600
11 20 15 15 2 100
12 17.5 17.5 22.5 3.5 3200
13 17.5 17.5 17.5 3.5 6400
14 17.5 17.5 17.5 0.5 1600
15 22.5 17.5 17.5 3.5 3200
16 15 20 15 2 1600
17 17.5 17.5 17.5 6.5 3200
18 17.5 22.5 17.5 3.5 1600
19 15 15 15 5 400
20 20 15 15 5 3200
21 15 20 15 5 1600
22 17.5 17.5 17.5 3.5 3200
23 12.5 17.5 17.5 3.5 800
24 17.5 17.5 17.5 3.5 3200
25 20 20 15 5 3200
26 17.5 17.5 12.5 3.5 800
27 17.5 12.5 17.5 3.5 3200
28 20 20 20 2 1600