Fig. 5.

Effect of tissue heterogeneity and proximity to tumor on Raman spectral parameters. Collagen mineralization by phosphate $\nu 1/\text{amide\hspace{0.17em}}\mathrm{I}$ (a) and phosphate $\nu 1/\text{proline}$ (b), and mineral crystallinity (d) showed significant differences only at the proximal positions to metaphysis in the tumor-bearing tibiae, while carbonation level (c) was enhanced at all measurement locations. The measurement locations on tibiae are shown in (e). (*$p<0.05$; **$p<0.01$; ***$p<0.001$).