TABLE 1.
SNF2h | Nucleosome | Analogue | Fraction Mobile | Keq (6.2mT/4.8 mT) | Splitting (mT) | Cone Angle |
---|---|---|---|---|---|---|
Wt | N+60 | ADP | 0.84 (0.83,0.85) | 0.19 | 4.77 | 117.5° |
Wt | N+60 | ADP* BeFx | 0.25 (0.21,0.28,0.25) | 3.1±0.6 | 6.22, 4.79 | 67.4°, 117.0° |
Wt | N+60 | ADP* AlFx | 0.19 (0.19,0.19) | 4.3 | 6.38, 4.73 | 61.2°, 119.1° |
Wt | None | ADP | 0.87 (0.89,0.84) | 0.16 | 4.72 | 119.1° |
Wt | None | ADP* BeFx | 0.94 (0.91,0.97) | 0.065 | 4.74 | 118.8° |
Wt | N | ADP* BeFx | 0.12 (0.11,0.17,0.1) | 7.4±2.2 | 6.29, 4.88 | 64.4°, 116.0° |
Wt | N | ADP* AlFx | 0.056 (0.04,0.07) | 19 | 6.46, 4.81 | 57.4°, 116.4° |
Wt | gH4+60 | ADP | 0.81 (0.78,0.85) | 0.24 | 4.76 | 118.1° |
Wt | gH4+60 | ADP* BeFx | 0.56 (0.50,0.56,0.63) | 0.8±0.2 | 6.31, 4.72 | 63.9°, 119.1° |
Wt | gH4+60 | ADP* AlFx | 0.74 (0.78,0.71) | 0.35 | 5.90, 4.73 | 79.6°, 120.3° |
Wt | bp+60 | ADP* BeFx | 0.61 (0.55,0.67) | 0.66 | 6.27, 4.80 | 65.6°, 116.8° |
Wt | 60mer DNA | ADP | 0.83, 0.82 (0.83) | 0.2 | 4.74 | 117.3° |
Wt | 60mer DNA | ADP* BeFx | 0.37 (0.32,0.41) | 1.8 | 6.28, 4.78 | 65.0°, 117.3° |
E309A | N+60 | ADP | 0.77 (0.8, 0.74) | 0.3 | 4.82 | 116.3° |
E309A | N+60 | ADP* BeFx | 0.79 (0.76, 0.81) | 0.27 | 4.78 | 117.7° |
E309A | N+60 | ADP* AlFx | 0.64 (0.8, 0.49) | 0.65 | 4.81 | 116.1° |