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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2014 Apr 18;18:18–30. doi: 10.1016/j.dnarep.2014.03.032

Figure 1. DNA substrates and purified Msh2-Msh3 and Msh2-msh3Y942A.

Figure 1

(a) The four different synthetic DNA substrates used in this study were homoduplex (non-specific), +8-loop (MMR), splayed Y and 3′flap (3′ NHTR) substrates. (b) Purified Msh2-Msh3 and Msh2-msh3Y942A (1.5 μg complex each) using the PBE94 purification (left) or the Q-Sepharose Fast Flow purification protocol (right). The protein complexes were analyzed by SDS-PAGE (8%) and stained with Coomassie Blue. Msh2 and Msh3 are indicated. The sizes of molecular weight markers (MW; Bio-Rad, broad range) are indicated alongside the gels in kDa.