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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2014 Apr 18;18:18–30. doi: 10.1016/j.dnarep.2014.03.032

Figure 6. Hydrolysis of ATP by Msh2-Msh3 was inhibited by ATPγS.

Figure 6

Increasing concentrations of ATPγS were titrated into a steady-state ATPase reaction in the presence of 50 nM protein and 250 nM DNA substrate, when present. The rates of hydrolysis were normalized to the rate in the absence of ATPγS (set at 100%). The dotted line in each panel represents the concentration of ATPγS required for 50% inhibition of ATP hydrolysis. The ATPγS titration was performed in the absence of DNA (a) or in the presence of homoduplex DNA (b), +8 loop DNA (c) or splayed Y DNA (d). (e) The concentrations of ATPγS required for 50% inhibition of Msh2-Msh3 ATPase activity in the presence of each DNA substrate.