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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2014 Apr 18;18:18–30. doi: 10.1016/j.dnarep.2014.03.032

Table 1.

ATP titrations to determine time dependence of ATP hydrolysis

ATPa (μM) Time (min.)b (A&B) Rate (μ M/min)c Time (min.) (A&B) Rate (μM/min)d
1A 1B 2A 2B
0 15.2e 0 0 16.1 0 0
5 NAf NA NA 26.1 −0.005 −0.005
10 NA NA NA 37.4 0.125 0.030
10 NA NA NA 46.9 0.058 0.069
10 NA NA NA 56.9 0.080 0.089
15 NA NA NA 67.3 0.150 0.153
30 NA NA NA 78.1 0.237 0.158
65 25.1 0.322 0.343 90.3 0.341 0.354
140 36.4 0.351 0.345 100.2 0.362 0.362
287.5 46.0 0.443 0.413 111.1 0.453 0.434
567.5 56.0 0.419 0.524 122.7 0.468 0.593
1125 66.4 0.645 0.610 132.0 0.633 0.631
a

Each titration was performed in duplicate (A and B). The reactions contained a fivefold excess of the +8 loop DNA substrate over Msh2-Msh3.

b

Total incubation time when rate was calculated.

c

Calculated rates for titration 1, starting at 65μM ATP.

d

Calculated rates for titration 2, starting at 5 μM ATP.

e

The time for replicate A is shown. The time for replicate B is less than 30 seconds later. It takes the reaction approximately 15 minutes to equilibrate.

f

Not applicable – measurements were not taken at these ATP concentrations.