. Author manuscript; available in PMC: 2014 Jun 16.

Published in final edited form as: Int J Biol Macromol. 2013 Mar 17;57:265–272. doi: 10.1016/j.ijbiomac.2013.03.026

Table 4.

¹³C and ¹H NMR spectroscopic data of Agaricus brasiliensis polysaccharides and its sulfated derivatives

Sample	Residue	Chemical shift (ppm)
Sample	Residue	C/H-1	C/H-2	C/H-3	C/H-4	C/H-5	C/H-6	C/H-6 term^c

FR	(1→6)-β-D- glucose	103.14 (4.60–4.73)	73.18 3.47	75.71 3.75	70.92, 68.97^b 3.68	75.03 3.88	69.60 (4.06–4.13)	63.34 (4.30–4.46)
FR-S	(1→6)-β-D- glucose	103.14 (4.72–4.78)	73.18 3.50	75.71 (3.70–3.90)	n.d. (76.07)^a, 68.95^b n.d. (4.65)^a	75.03 (4.00–4.22)	69.60 4.39	n.d. (71.59)^a n.d. (5.32)^a
FR	(1→3)-β-D- glucose	103.14 (4.96, 4.94)	73.18 3.86	n.d. (3.94, 3.96)	69.35 3.79	75.71 3.77	60.69 (3.90, 3.91)	63.34 (4.20–4.29)
FR-S	(1→3)-β-D- glucose	103.14 (4.85, 4.90)	73.18 (3.70–3.90)	n.d. (4.00–4.22)	n.d. (74.25)^a n.d. (4.59)^a	75.71 (3.70–3.90)	60.69 3.90 (5.07)^a	n.d. (71.59)^a n.d. (5.27)^a
MI	(1→2)-β-D- mannose	104.88, 98.55 5.30	78.59 (3.20–4.00)	74.11 (3.20–4.00)	72.26 (3.20–4.00)	78.40 (3.20–4.00)	63.25, 3.40 (3.20–4.00)	60.08 (3.20–4.00)
MI-S	(1→2)-β-D- mannose	105.71, 98.48 5.35	78.63 (3.80–4.10)	74.00 (78.91–79.69)^a 3.70 (4.30)^a	72.05 (77.64)^a 3.60 (4.20)^a	78.33 3.37	63.21 (70.43)^a (3.80–4.10) (5.46)^a	n.d. (69.10)^a (3.80–4.10) (5.92)^a
MI	(1→3)-β-D- glucose	102.56, 94.74 (4.60–4.90)	75.40 (3.20–4.00)	n.d. (3.20–4.00)	72.00 (3.20–4.00)	76.79 (3.20–4.00)	63.40 (3.20–4.00)	60.08 (3.20–4.00)
MI-S	(1→3)-β-D- glucose	102.27, 94.60 (4.60–4.90)	75.59 (80.87–80.93)^a 3.60 (4.20)^a	86.71 (3.80–4.10)	71.25 (77.28)^a 3.48 (4.13)^a	76.72 3.37	63.41 (70.55)^a (3.80–4.10) (5.85)^a	n.d. (69.10)^a (3.80–4.10) (5.92)^a

FR and FR-S, A. brasiliensis fruiting body polysaccharide and its sulfated derivative.

MI and MI-S, A. brasiliensis mycelial polysaccharide and its sulfated derivative.

Assignments corresponding to the respective sulfation sites are shown in bold font between parentheses.

Assignment for the C4 of (1→6)-β-D-glucose linked to the side chain in C3.

term.: corresponding to the terminal sugar residues.

n.d.: not detected.

Chemical shifts were assigned using the CASPER program [60] and according to previously published data [16, 35, 42, 52].