. Author manuscript; available in PMC: 2015 May 15.

Published in final edited form as: Arch Biochem Biophys. 2014 Apr 5;0:1–11. doi: 10.1016/j.abb.2014.03.013

Table 1.

Numerical results from fitting of the Hill equation (Eq. 1) to Ca²⁺ titration curves for each pseudo-phosphorylation mutant and control. Parameter standard error is given to the right of each parameter value.

cTnI:cTnT Mutants (n = 5–10)	Thin filament		Thin filament + S1-ADP

	pCa₅₀	n_H	pCa₅₀	n_H
S151C_AEDANS:wt	6.27 ± 0.01	0.786 ± 0.014	6.50 ± 0.01	0.765 ± 0.014
S199E	6.29 ± 0.02	0.789 ± 0.019	6.49 ± 0.02	0.744 ± 0.014
T204E	6.09 ± 0.02	0.853 ± 0.01	6.22 ± 0.01	0.716 ± 0.018
S199E/T204E	6.18 ± 0.02	0.731 ± 0.012	6.28 ± 0.02	0.714 ± 0.014
T195E/S199E/T204E	6.36 ± 0.03	0.831 ± 0.017	6.43 ± 0.01	0.741 ± 0.016
T195E/S199E/T204E/T285E	6.47 ± 0.03	0.711 ± 0.019	6.53 ± 0.02	0.683 ± 0.022
S167C_AEDANS:wt	6.11 ± 0.02	1.03 ± 0.03	6.28 ± 0.01	0.773 ± 0.017
S199E	6.03 ± 0.01	0.881 ± 0.024	6.26 ± 0.02	0.826 ± 0.018
T204E	5.98 ± 0.02	1.14 ± 0.04	6.12 ± 0.01	0.843 ± 0.016
S199E/T204E	6.11 ± 0.01	1.13 ± 0.04	6.31 ± 0.02	0.705 ± 0.018
T195E/S199E/T204E	6.17 ± 0.01	1.11 ± 0.03	6.28 ± 0.01	0.759 ± 0.017
T195E/S199E/T204E/T285E	6.21 ± 0.01	0.924 ± 0.013	6.29 ± 0.02	0.863 ± 0.019