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. Author manuscript; available in PMC: 2015 May 15.

Published in final edited form as: Arch Biochem Biophys. 2014 Apr 5;0:1–11. doi: 10.1016/j.abb.2014.03.013

Table 2.

Ca²⁺ and S1-dependent changes in distance between cTnI(S151C_AEDANS) and cTnC(S89C_DDPM) in reconstituted cardiac thin filaments.

cTnI:cTnT Mutants (n=4–6)	Thin filament + Mg²⁺		Thin filament + S1-ADP + Mg²⁺

	r̄	hw	r̄	hw

S151C_AEDANS:WT	41.2 ± 2.0	15.0 ± 2.2	39.5 ± 3.8	12.6 ± 4.6
S199E	40.7 ± 2.0	14.7 ± 2.2	39.0 ± 2.7	12.3 ± 3.2
T204E	40.3 ± 2.4	14.2 ± 2.4	38.4 ± 3.0	11.1 ± 3.5
S199E/T204E	39.9 ± 2.6	13.7 ± 2.9	38.9 ± 2.7	12.0 ± 2.9
T195E/S199E/T204E	38.8 ± 2.1^*	12.7 ± 2.5^*	37.9 ± 3.3	10.9 ± 3.7
T195E/S199E/T204E/T285E	38.2 ± 2.8^*	12.0 ± 3.1^*	37.2 ± 3.3^*	10.1 ± 3.5

	Thin filament + Ca²⁺		Thin filament + S1-ADP + Ca²⁺

	r̄	hw	r̄	hw

S151C_AEDANS:WT	35.2 ± 1.3	10.3 ± 3.4	35.3 ± 2.4	8.4 ± 2.8
S199E	34.8 ± 1.4	9.67 ± 3.1	34.8 ± 1.9	8.3 ± 1.9
T204E	35.1 ± 1.7	8.68 ± 1.8	34.7 ± 2.2	8.2 ± 2.4
S199E/T204E	34.8 ± 1.5	8.41 ± 1.7	34.8 ± 2.2	8.5 ± 2.1
T195E/S199E/T204E	34.4 ± 1.6	7.90 ± 1.8	34.2 ± 2.3	7.7 ± 2.4
T195E/S199E/T204E/T285E	34.1 ± 2.1^*	7.70 ± 2.3	33.6 ± 3.0^*	7.1 ± 3.4

^*

Indicates a significant difference from wild type at the 90% level while

^**

indicates significance at the 95% level.