. Author manuscript; available in PMC: 2015 May 15.

Published in final edited form as: Arch Biochem Biophys. 2014 Apr 5;0:1–11. doi: 10.1016/j.abb.2014.03.013

Table 4.

Deactivation rate parameters obtained from exponential fitting of FRET kinetic transients ± 90% confidence interval. Rate constants for thin filament samples with S1-ADP are given in the right column. The top half of the table shows results obtained from cTnI(151C_AEDANS)-cTnC(89C_DDPM) FRET pairs, while the bottom half show results obtained from cTnI(167C_AEDANS)-cTnC(89C_DDPM) pairs.

cTnI:cTnT mutants (n = 20–60)	Kinetic rate (s⁻¹)

	Thin filament	Thin filament +S1- ADP
S151C_AEDANS:wt	34.1 ± 1.9	33.6 ± 2.1
S199E	32.5 ± 2.0	30.2 ± 2.1^*
T204E	34.5 ± 2.8	32.8 ± 2.7
S199E/T204E	35.1 ± 2.2	32.3 ± 2.7
T195E/S199E/T204E	32.8 ± 2.9	31.8 ± 2.8
T195E/S199E/T204E/T285E	32.2 ± 2.2	32.1 ± 2.4
S167C_AEDANS:wt	46.3 ± 3.3	39.9 ± 4.0
S199E	37.9 ± 2.7^**	36.9 ± 2.9
T204E	36.1 ± 2.5^**	37.0 ± 2.2
S199E/T204E	39.1 ± 3.3^**	35.7 ± 2.7
T195E/S199E/T204E	38.8 ± 4.1^**	35.7 ± 3.4
T195E/S199E/T204E/T285E	35.1 ± 2.6^**	34.9 ± 3.3

Indicates a significant difference from wild type at the 90% confidence level while

^**

indicates significance at the 95% level.