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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Xenobiotica. 2013 Sep 6;44(4):309–319. doi: 10.3109/00498254.2013.835885

Figure 3.

Figure 3

Identification of m/z 316 isomeric products. Extracted ion chromatograms (m/z 316) of CYP3A4 metabolite samples after (A) no treatment, (B) HCl treatment only, or (C) TiCl3 in HCl treatment. Note that after treatment with HCl and TiCl3, M4b retention time was slightly shifted to ~20 min; however, all fragmentation patterns matched those for M4b eluting at ~21 min in the control sample. (D) Corresponding MS2 for m/z 316 isomeric product with retention time of ~19 min, M4a. (E) Corresponding MS2 for m/z 316 isomeric product with retention time of ~21 min, M4b. (F) Corresponding MS2 for m/z 316 isomeric product with retention time of ~23 min, M4c. (G) Corresponding MS3 for m/z 316 isomeric product M4a with retention time of ~19 min and MS2 of 243. (H) Corresponding MS3 for m/z 316 isomeric product M4b with retention time of ~21 min and MS2 of 243. (I) Corresponding MS3 for m/z 316 isomeric product M4c with retention time of ~23 min and MS2 of 227. Data shown are representative of those found in a minimum of four separate experiments.