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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Stem Cells. 2008 Jun 5;26(8):2131–2141. doi: 10.1634/stemcells.2008-0388

Figure 4.

Figure 4

Histone methyltransferase G9a restricts Oct4-EGFP reactivation during ESC-induced reprogramming. (A): Expression of G9a in ESCs and adult neural stem cells/progenitors (NSCs). Shown is a summary of quantitative real-time polymerase chain reaction analysis of the expression level of G9a in Z-Red ESCs, CIPOE NSCs, CIPOE NSCs with shCon, and shG9a. The mRNA abundance was normalized to the levels in CIPOE cells. Values represent mean ± SEM (n = 3; *, p < .01, Student’s t test). (B): Summary of reprogramming frequencies. Shown are results from cell fusion experiments between Z-Red and CIPOE, CIPOE-shCon, or CIPOE-shG9a cells. Values represent mean ± SEM (n = 3; *, p < .01, Student’s t test). (C): Summary of reprogramming efficacy. Cumulative distribution plots of DsRed+ population with graded EGFP fluorescence intensities are shown for comparison of the reprogramming efficacy of Z-Red ESCs with CIPOE-shCon, or CIPOE-shG9a cells at days 2 and 4 after cell fusion. Values represent mean ± SEM (n = 3; *, p < .01, Kolmogorov-Smirnov test). Abbreviations: EGFP, enhanced green fluorescent protein; shCon, control short hairpin RNA; shG9a, G9a-targeted short hairpin RNA.