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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Stem Cells. 2008 Jun 5;26(8):2131–2141. doi: 10.1634/stemcells.2008-0388

Figure 6.

Figure 6

Synergistic enhancement of reprogramming by a combination of Jhdm2a with the pluripotency-specific transcription factor Nanog. (A): A schematic drawing of the model on mechanisms underlying ESC fusion-induced Oct4-EGFP reactivation during reprogramming of somatic neural stem cells/progenitors. (B): Summary of reprogramming frequencies. Shown are results from cell fusion experiments between Z-Red and CIPOE, CIPOE-Nanog, or CIPOE-Nanog plus Jhdm2a cells. Values represent mean ± SEM (n = 3; *, p < .01, Student’s t test). (C): Summary of reprogramming efficacy. Cumulative distribution plots of DsRed+ population with graded EGFP fluorescence intensities are shown for comparison of the reprogramming efficacy of Z-Red ESCs with CIPOE, CIPOE-Jhdm2a, or CIPOE-Nanog plus Jhdm2a cells at day 4 after cell fusion. Values represent mean ± SEM (n = 3; *, p < .01, Kolmogorov-Smirnov test). Abbreviations: EGFP, enhanced green fluorescent protein; H3K9diM, histone H3 lysine 9 dimethylation.