Table 1.
Validation of previously identified Chagas biomarkers within HUG study samples
| m/z (/1,000) | Fractions and chemistries | P value (CD + vs HC) AUC for ROC curve (fold CD/HC) | P value (FU vs HC) AUC for ROC curve (fold FU/HC) | P value (CD + vs FU) AUC for ROC curve (fold CD/FU) |
Mean signal intensity ± SEM |
||
|---|---|---|---|---|---|---|---|
|
HC |
CD+ |
FU |
|||||
| (n = 37) | (n = 37) | (n = 37) | |||||
| 3.7 |
F1 CM10 |
0.0001, 1.0 (+4.2) |
0.0001, 0.88 (+2.3) |
0.0011, 0.82 (+1.8) |
1.54 ± 0.11 |
6.46 ± 0.64 |
3.60 ± 0.45 |
| 4.4 |
F1 IMAC; F1,5 CM10 |
0.0001, 1.0 (+3.7) |
0.0159, 0.69 (+1.7) |
0.0001, 0.91 (+2.2) |
7.66 ± 0.53 |
28.35 ± 1.74 |
12.84 ± 1.91 |
| 5.4a |
F1, 3 CM10 |
0.0001, 1.0 (-0.3) |
0.0001, 0.99 (-0.2) |
0.2427, 0.65 (+1.2) |
3.51 ± 0.31 |
0.90 ± 0.07 |
0.75 ± 0.10 |
| 6.6 |
F3,5 CM10 |
0.0001, 1.0 (+3.4) |
0.0001, 0.91 (+2.5) |
0.0028, 0.79 (+1.4) |
3.65 ± 0.26 |
12.45 ± 0.62 |
8.96 ± 0.89 |
| 7.8 |
F1,3 CM10 |
0.0001, 0.97 (+3.9) |
0.3405, 0.55 (+1.2) |
0.0005, 0.91 (+3.15) |
0.55 ± 0.07 |
2.14 ± 0.35 |
0.68 ± 0.12 |
| 9.3 |
F1,4 IMAC; F1,3 CM10 |
0.0001, 0.92 (+1.7) |
0.9456, 0.51 (+1.0) |
0.0001, 0.87 (+1.7) |
2.74 ± 0.26 |
4.76 ± 0.24 |
2.77 ± 0.31 |
| 10.1 |
F1,3,4,6 IMAC; F1 CM10 |
0.0001, 0.88 (+2.0) |
0.3622, 0.56 (-0.9) |
0.0001, 0.96 (+2.3) |
15.27 ± 1.80 |
30.48 ± 2.92 |
13.13 ± 1.42 |
| 12.7 |
F2,5 IMAC; F1,5,6 CM10 |
0.0003, 0.80 (+2.0) |
0.5879, 0.53 (+1.2) |
0.0129, 0.73 (+1.7) |
2.59 ± 0.41 |
5.27 ± 0.55 |
3.02 ± 0.68 |
| 13.6 |
F1 IMAC; F1 CM10 |
0.0001, 0.96 (+2.3) |
0.2220, 0.59 (+1.2) |
0.0001, 0.86 (+1.8) |
4.43 ± 0.51 |
10.07 ± 0.62 |
5.50 ± 0.71 |
| 15.2 |
F4,5 IMAC; F1,3,6 CM10 |
0.0001, 1.0 (+2.9) |
0.6538, 0.51 (+1.1) |
0.0001, 0.96 (+2.7) |
5.62 ± 0.51 |
16.46 ± 1.33 |
6.04 ± 0.75 |
| 16.3 |
F4 IMAC; F3,6 CM10 |
0.0001, 0.95 (+3.1) |
0.4349, 0.58 (+1.2) |
0.0001, 0.94 (+2.6) |
1.77 ± 0.30 |
5.48 ± 0.48 |
2.10 ± 0.28 |
| 24.7 |
F2 IMAC |
0.0001, 0.84 (+1.8) |
0.6429, 0.51 (-0.9) |
0.0001, 0.85 (+1.9) |
1.48 ± 0.16 |
2.61 ± 0.19 |
1.37 ± 0.18 |
| 28.1a |
F1,3 CM10 |
0.0001, 0.96 (-0.8) |
0.3598, 0.56 (-1.0) |
0.0032, 0.79 (-0.8) |
28.43 ± 0.63 |
21.66 ± 1.07 |
27.06 ± 1.23 |
| 28.9 |
F1 IMAC |
0.0001, 0.98 (+3.5) |
0.0182, 0.66 (+1.9) |
0.0006, 0.80 (+1.9) |
0.46 ± 0.07 |
1.61 ± 0.13 |
0.86 ± 0.15 |
| 75.4 | F1 CM10 | 0.0001, 1.0 (+3.0) | 0.0005, 0.83 (+1.9) | 0.0001, 0.96 (+1.6) | 1.18 ± 0.15 | 3.53 ± 0.09 | 2.25 ± 0.23 |
a Downregulated protein in CD-positive subjects.
Mass (m/z), mean intensities, and AUC’s for selected differentially expressed peptides/proteins between HC, 2008-CD + and 2011-FU sera samples.
An area under the curve (AUC) of 1 suggests good diagnostic power, while AUC values that approach 0.5 indicate little diagnostic potential. HC: Healthy control; CD: Chagas disease; FU: follow-up.