Table 4.

Effect of METS 50, 100 and 150 mg/kg in Tail immersion test

Samples	Dose mg/kg	Tail withdrawing time in sec
		0 min	30 min	60 min	90 min	120 min
1% tween 80	10 ml/kg	3.45 ± 0.26	3.48 ± 0.19ns	3.27 ± 0.032ns	3.48 ± 0.20ns	3.57 ± 0.19
METS	50 mg/kg	3.48 ± 0.19	3.57 ± 0.19ns	4.11 ± 0.12ns	4.16 ± 0.12ns	4.09 ± 0.11ns
	100 mg/kg	3.53 ± 0.18	4.25 ± 0.04ns	4.49 ± 0.13ns	4.39 ± 0.08ns	4.29 ± 0.04ns
	150 mg/kg	3.57 ± 0.19	4.82 ± 0.04ns	5.48 ± 0.02ns	5.27 ± 0.05ns	5.18 ± 0.06ns
TramadolR	30 mg/kg	3.48 ± 0.20	4.99 ± 0.33	5.69 ± 0.02	5.49 ± 0.06ns	5.38 ± 0.05ns
Antinociceptive effect of METS and TramadolR antagonized by Naloxone
METS	150 mg/kg	3.52 ± 0.02 ns	2.64 ± 0.01ns	2.87 ± 0.02ns	2.73 ± 0.01ns	2.69 ± 0.01ns
TramadolR	30 mg/kg	3.50 ± 0.02	2.35 ± 0.02	2.69 ± 0.01	2.75 ± 0.02	2.65 ± 0.02

^nsValues statistically non significant when compared to control using one way ANOVA followed by Dunnett’s multiple comparison test.