Table 1.
Characteristics of cfr -carrying isolates and transformants
|
Isolate |
Staphylococcal species |
Origin |
Market |
PFGE type
a
|
Location of
cfr
b
|
MIC values of antimicrobial agents (mg/L)
c
|
Other resistance patterns
d
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CHL | FFC | CLR | TIA | VAL | LZD | |||||||
| TDP5 |
S. cohnii |
Pork |
1 |
C |
P |
16 |
>64 |
>64 |
128 |
64 |
2 |
OXA, CIP, GEN, ERY, TET |
| TDPJC2 |
S. cohnii |
Chicken |
1 |
P |
ND |
32 |
32 |
>64 |
64 |
0.5 |
2 |
OXA, CIP, ERY |
| TYT5 |
S. cohnii |
Pork |
3 |
F |
P |
32 |
32 |
64 |
128 |
64 |
2 |
TET |
| TYT7 |
S. cohnii |
Pork |
3 |
F |
P |
16 |
>64 |
>64 |
64 |
16 |
2 |
OXA, CIP, GEN, ERY |
| TDP9 |
S. equorum |
Pork |
1 |
D |
P |
32 |
>64 |
>64 |
>128 |
>64 |
8 |
OXA, GEN, ERY, TET |
| TDPJC9 |
S. equorum |
Chicken |
1 |
J |
P |
16 |
64 |
>64 |
128 |
2 |
4 |
OXA, GEN, ERY, TET |
| TLD18 |
S. equorum |
Pork |
2 |
L1 |
P |
16 |
>64 |
>64 |
>128 |
64 |
8 |
OXA, GEN, ERY, TET |
| TLDJC5 |
S. equorum |
Chicken |
2 |
L2 |
P |
64 |
32 |
>64 |
>128 |
16 |
4 |
OXA, CIP, GEN, ERY, RIF, TET |
| TLDJC9 |
S. equorum |
Chicken |
2 |
N |
P |
32 |
64 |
>64 |
>128 |
2 |
4 |
OXA, CIP, GEN, ERY, RIF, TET |
| TLH5 |
S. equorum |
Pork |
4 |
L3 |
ND |
16 |
>64 |
>64 |
128 |
8 |
4 |
OXA, CIP, GEN, ERY, TET |
| TYTJC3 |
S. equorum |
Chicken |
3 |
I |
ND |
16 |
32 |
>64 |
>128 |
4 |
4 |
ERY, TET |
| TYTJC8 |
S. equorum |
Chicken |
3 |
I |
ND |
16 |
32 |
64 |
64 |
16 |
2 |
OXA, CIP, GEN, ERY |
| TDPJC13 |
S. sciuri |
Chicken |
1 |
E |
P |
64 |
64 |
>64 |
>128 |
32 |
4 |
OXA, CIP, GEN, TET |
| TDPJC5 |
S. sciuri |
Chicken |
1 |
R |
ND |
32 |
>64 |
>64 |
>128 |
>64 |
16 |
OXA, GEN, ERY, TET |
| TLKJC2 |
S. sciuri |
Chicken |
6 |
Q |
P |
16 |
>64 |
>64 |
>128 |
16 |
8 |
OXA, CIP, GEN, ERY, TET |
| TDP12 |
S. simulans |
Pork |
1 |
A |
ND |
>64 |
64 |
64 |
64 |
16 |
4 |
OXA, CIP, GEN, ERY, RIF |
| TDP24 |
S. simulans |
Pork |
1 |
B |
ND |
32 |
>64 |
>64 |
64 |
2 |
4 |
TET |
| THTJC2 |
S. simulans |
Chicken |
5 |
O |
P |
64 |
32 |
>64 |
>128 |
4 |
4 |
OXA, CIP, GEN, ERY, RIF |
| TLD12 |
S. simulans |
Pork |
2 |
K |
P |
>64 |
>64 |
>64 |
>128 |
64 |
8 |
OXA, CIP, GEN, ERY, RIF, TET |
| TLD20 |
S. simulans |
Pork |
2 |
M |
P |
>64 |
>64 |
>64 |
128 |
32 |
4 |
OXA, CIP, GEN, ERY, RIF, TET |
| TLD22 |
S. simulans |
Pork |
2 |
G2 |
P |
16 |
>64 |
>64 |
128 |
8 |
8 |
CIP, GEN, ERY, TET |
| TYT6 |
S. simulans |
Pork |
3 |
G1 |
ND |
16 |
>64 |
>64 |
>128 |
64 |
4 |
OXA, ERY, TET |
| Recipient RN4220 |
S. aureus |
|
|
|
|
4 |
4 |
0.25 |
0.5 |
0.25 |
1 |
ND |
| RN4220-pHNLKJC2 |
S. aureus |
|
|
|
|
32 |
64 |
16 |
16 |
8 |
4 |
ND |
| DH5α |
E. coli |
|
|
|
|
4 |
4 |
- |
- |
- |
- |
ND |
| DH5α-pUC18-cfr |
E. coli |
|
|
|
|
8 |
8 |
- |
- |
- |
- |
ND |
| ATCC 29213 | S. aureus | 2 | 2 | 0.12 | 0.5 | 0.06 | 1 | |||||
aPatterns that differed from pattern A by six or more bands were considered to represent different strains. Patterns that differed by fewer than six bands were considered to represent subtypes within the main group (e.g.,L1, L2).
bP, plasmid; ND, not determined.
cCHL, chloramphenicol; FFC, florfenicol; CLR, clindamycin; TIA, tiamulin; VAL, valnemulin; LZD, linezolid. MIC was not measured because of known intrinsic resistance or naturally high MICs.
dThe results were interpreted according to Eucast breakpoints ( http://www.eucast.org/clinical_breakpoints/). OXA, oxacillin; CIP, ciprofloxacin; GEN, gentamycin; ERY, erythromycin; RIF, rifamycin; TET, tetracycline. All isolates were susceptible to vancomycin. ND, not determined.