. Author manuscript; available in PMC: 2014 Jun 16.

Published in final edited form as: Arch Biochem Biophys. 2013 Apr 4;535(2):143–149. doi: 10.1016/j.abb.2013.03.012

Table 1.

Summary of kinetic parameters of propofol glucuronidation in human hepatocytes with and without siRNA treatment. Intrinsic clearance values (Clint) were calculated by dividing the mean V_max parameter by the mean K_m parameter for each treatment group. OPTI Only and TR only refer to control incubations containing only Optimem assay buffer or Optimem buffer plus transfection reagent, respectively. Statistical significance is calculated versus the transfection reagent only control

	V_max (μmol/min)	K_m (μM)	K_i (μM)	Cl_int (mL/min)
OPTI Only	0.393 ± 0.009	7.99 ± 0.45	361.7 ± 31.3	49.1
TR Only	0.277 ± 0.005	7.62 ± 0.37	521.2 ± 46.3	36.3
UGT1A9 siRNA	0.073 ± 0.007^*	6.02 ± 1.6^‡	448.4 ± 203.5^‡	12.1^*
UGT2B7 siRNA	0.161 ± 0.027^†	10.5 ± 3.7^‡	198.5 ± 88.9^†	15.3^*

p < 0.0001;

^†

p < 0.005;

^‡

p > 0.1, no significant difference