Figure 1. Aminopeptidases in the human urinary proteome.

A. Gender-specific proteomic analysis (this work). Pooled female and male urine samples were separately analyzed by LC-MS/MS of tryptic peptides, before and after immuno-depletion of 14 abundant plasma proteins (see section 2.2, supplementary methods and materials, and supplementary proteomics data). The number of proteins uniquely detected (2-peptide minimum) in each gender, as well as those in common, are shown in the Venn diagram. Identified exopeptidases are indicated.

B. Comparison of three urinary proteomic analyses. Exopeptidases positively identified by Adachi et al [26], Marimuthu et al [27] and in this study are shown in the Venn diagram. Note that the published urinary proteomes are of mixed gender composition. The five aminopeptidases detected in all three studies were taken for further functional assays.