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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Biochim Biophys Acta. 2012 Nov 1;1827(3):365–386. doi: 10.1016/j.bbabio.2012.10.012

Figure 7. SQ occupancy at the Qo-site in wildtype and E295W mutant.

Figure 7

The green curve shows the CW-EPR derivative spectrum of the Qo-site SQ, obtained by subtraction of the signal when ascochlorin was added to the reaction mixture so as to remove components not associated with Qo-site turnover. The blue curve shows the result of a first integration (to generate the absorbance spectrum), and the red curve a second integration to determine the occupancy, shown by the right-hand scale. Antimycin was present in all experiments to eliminate contributions from the Qi-site SQ. See Materials and Methods section for details.