Skip to main content
. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Dev Biol. 2011 Sep 16;360(1):66–76. doi: 10.1016/j.ydbio.2011.09.001

Figure 2. Traf3ip1 mutant mice are embryonic lethal and display phenotypes typically associated with defects in ciliogenesis.

Figure 2

(A) Schematic of the Traf3ip1 wildtype (Traf3ip1 WT) and Traf3ip1 mutant gene trap allele (Traf3ip1 GT). (B) Real-time reverse transcriptase PCR results for the expression of Traf3ip1 in homozygous Traf3ip1 WT and Traf3ip1 GT mouse embryonic fibroblasts. Expression levels are normalized to the internal control levels of GAPDH and relative to the wildtype level of Traf3ip1. (C) The heads of a Traf3ip1 WT and (D) Traf3ipGT mutant embryos at day 10.5 (E10.5) show significant neural developmental defects as well as (D, arrow) microphthalmia in the Traf3ip1 GT mutant. (E and G) Traf3ip1 WT embryos on the left and Traf3ip1GT mutant embryos on the right. (E, arrow) Significant cardiac edema is observed in the embryonic day 13.5 mutant embryo. (G) A curled tail phenotype associated with E10.5 Traf3ip1GT mutant embryos. (F) A polydactylous forelimb of an embryonic day 13.5 Traf3ip1 GT mutant embryo.