Skip to main content
. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Dev Biol. 2011 Sep 16;360(1):66–76. doi: 10.1016/j.ydbio.2011.09.001

Figure 4. Traf3ip mutant cells display an elevated mTor response to serum.

Figure 4

(A) Traf3ip1 WT and Traf3ip1GT mutant MEF cells were cultured for 48 hours with no serum and then exposed to serum for 5, 10, and 30 minutes prior to total protein isolation and western blotting for the following mTor pathway proteins and their phosphorylated forms: phospho-mTor (p-mTor), total mTor (mTor), phospho-p70 S6 kinase (p-p70 S6K), total p70 S6 kinase (p70 S6K), phospho- PRAS40 (p-PRAS40), total PRAS40 (PRAS40), phospho-S6 ribosomal protein serines 235/236 (p-S6 235/236), phospho-S6 ribosomal protein serines 240/244 (p-S6 240/244), total S6 ribosomal protein (S6), and loading control the Golgi marker GM130 (GM130). (B) Densitometry analysis of the serum time course, results for fold changes between the total protein and its phosphorylated form(s), are shown for mTor, PRAS40, p70S6 Kinase, S6 ribosomal protein both the serine phosphorylation at sites 235/236 and 240/244.