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. 2014 Jun 16;9(6):e98994. doi: 10.1371/journal.pone.0098994

Table 8. Estimation of time and diving effort needed for data acquisition within each box used in the EBQI.

Box Proxy Time Dive organization
1 – P. oceanica roots and rhizomes 30 random in situ measures (growth of the rhizome corresponding to the last 8 leaf bases) 30 min 1 dive for 2 scientific divers
2 - P. oceanica leaves 20 random measures of shoot density in a square frame (0.16 m2) 60 min
30 random measures of cover 10 min
3 - MPO leaf epibiota Sampling of the two oldest external leaves on 30 shoot randomly localized to estimate the epibiota biomass 10 min
4 - Filter- and suspension-feeder leaf epibiota Sampling of the two oldest external leaves on 30 shoot randomly localized to estimate the epibiota biomass Same as box 3
5 – Filter feeder benthic epibiota Density of Pinna nobilis along 20 transects 10-m long and 1-m wide 120 min 1 dive for 2 scientists divers
7 - Litter detritus Litter detritus mass collected in July, within 5 randomly localized 0.1-m2 quadrats 90 min 1 dive for 2 scientist divers
6 – Other filter- and suspension-feeder benthic epibiota Abundance of filter- and suspension-feeder benthic epibiota other than Pinna nobilis within 1-m2 quadrats, with 30 replicates randomly localized 120 min 1 dive for 2 scientist divers
8 - Detritus feeders Abundance of Holothuria spp. within 1-m2 quadrats, with 30 replicates randomly localized Same as box 6
9 - Herbivores 1 Abundance of Paracentrotus lividus within 1-m2 quadrats, with 30 replicates randomly localized at 5 m depth 60 min 1 dive for 2 scientist divers
Percentage of intermediate and adult leaves exhibiting bite scars due to S. salpa (all the intermediate and adult leaves of 30 shoots randomly localized) at 5 m depth 30 min
10 - Predatory teleosts All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes. 60 min 1 dive for 2 scientist divers
11 - Piscivorous teleosts All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes Same as boxes 10 and 12
12 - Planktivorous teleosts of the water column All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes Same as boxes 10 and 11
9–12 - SRDI All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes Same as boxes 10, 11 and 12