1 – P. oceanica roots and rhizomes |
30 random in situ measures (growth of the rhizome corresponding to the last 8 leaf bases) |
30 min |
1 dive for 2 scientific divers |
2 - P. oceanica leaves |
20 random measures of shoot density in a square frame (0.16 m2) |
60 min |
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30 random measures of cover |
10 min |
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3 - MPO leaf epibiota |
Sampling of the two oldest external leaves on 30 shoot randomly localized to estimate the epibiota biomass |
10 min |
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4 - Filter- and suspension-feeder leaf epibiota |
Sampling of the two oldest external leaves on 30 shoot randomly localized to estimate the epibiota biomass |
Same as box 3 |
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5 – Filter feeder benthic epibiota |
Density of Pinna nobilis along 20 transects 10-m long and 1-m wide |
120 min |
1 dive for 2 scientists divers |
7 - Litter detritus |
Litter detritus mass collected in July, within 5 randomly localized 0.1-m2 quadrats |
90 min |
1 dive for 2 scientist divers |
6 – Other filter- and suspension-feeder benthic epibiota |
Abundance of filter- and suspension-feeder benthic epibiota other than Pinna nobilis within 1-m2 quadrats, with 30 replicates randomly localized |
120 min |
1 dive for 2 scientist divers |
8 - Detritus feeders |
Abundance of Holothuria spp. within 1-m2 quadrats, with 30 replicates randomly localized |
Same as box 6 |
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9 - Herbivores 1 |
Abundance of Paracentrotus lividus within 1-m2 quadrats, with 30 replicates randomly localized at 5 m depth |
60 min |
1 dive for 2 scientist divers |
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Percentage of intermediate and adult leaves exhibiting bite scars due to S. salpa (all the intermediate and adult leaves of 30 shoots randomly localized) at 5 m depth |
30 min |
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10 - Predatory teleosts |
All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes. |
60 min |
1 dive for 2 scientist divers |
11 - Piscivorous teleosts |
All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes |
Same as boxes 10 and 12 |
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12 - Planktivorous teleosts of the water column |
All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes |
Same as boxes 10 and 11 |
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9–12 - SRDI |
All teleosts counted within ten linear 50-m long and 5-m wide transects, each census lasting 5 minutes |
Same as boxes 10, 11 and 12 |
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