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. 2014 Jun 16;9(6):e97629. doi: 10.1371/journal.pone.0097629

Table 1. Primers used in the current study.

Primer name Primer sequence (5′-3′) rRNA operon binding site Reference
ITS1F (F) CTTGGTCATTTAGAGGAAGTAA Small subunit [19]
ITS2 (R) GCTGCGTTCTTCATCGATGC 5.8S [18]
ITS3 (F) GCATCGATGAAGAACGCAGC 5.8S [18]
ITS4 (R) TCCTCCGCTTATTGATATGC Large subunit [18]
ITS86F (F) GTGAATCATCGAATCTTTGAA 5.8S [27]
ITS86R (R) TTCAAAGATTCGATGATTCAG 5.8S [26]

Primers are indicated as forward (F) or reverse (R). ITS86R contains a wrong base at the 3′ end. The G should be replaced by a C (see Discussion).