Table 1. Primers used in the current study.
Primer name | Primer sequence (5′-3′) | rRNA operon binding site | Reference |
ITS1F (F) | CTTGGTCATTTAGAGGAAGTAA | Small subunit | [19] |
ITS2 (R) | GCTGCGTTCTTCATCGATGC | 5.8S | [18] |
ITS3 (F) | GCATCGATGAAGAACGCAGC | 5.8S | [18] |
ITS4 (R) | TCCTCCGCTTATTGATATGC | Large subunit | [18] |
ITS86F (F) | GTGAATCATCGAATCTTTGAA | 5.8S | [27] |
ITS86R (R) | TTCAAAGATTCGATGATTCAG | 5.8S | [26] |
Primers are indicated as forward (F) or reverse (R). ITS86R contains a wrong base at the 3′ end. The G should be replaced by a C (see Discussion).